Environmentally Benign Staining Procedure for Electrophoresis Gels Using Coomassie Brilliant Blue

Environmentally benign staining procedure for electrophoresis gels using Coomassie Brilliant Blue.

Rapid Coomassie blue staining and destaining of polyacrylamide gels.

Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.

A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-media...

Repeated probing of western blots obtained from coomassie brilliant blue-stained or unstained polyacrylamide gels.

For several reasons, it can be desirable to probe a filter obtained after Western blotting of polyacrylamide gels with different antibodies. On the one hand, protein amounts may be limiting and re-probing of a protein filter may be the only way to identify different antigens. On the other hand, in the case of two-dimensional (2-D) gel electrophoresis, exact reproducibility of the protein patter...

A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies.

Proteins stained with Coomassie Brilliant Blue I were transferred effectively by blotting from polyacrylamide gel electrophoresis (PAGE) gels to transparencies of the type used in plain-paper copiers. The details of the original electropherogram were retained on transfer and did not fade over a period of three years. Both the protein and the associated dye transfer; however, protein does not tr...